HunterThinks.com

见死不救

Posted: 14 January 2018
Updated: 14 January 2018

The cost to make my cure, update #1

Bottom line: if you kindly send me US$65, I will make more progress towards recovering.

Psilocybin can cure my health problems, so I am making it. Ten days ago, I projected that to buy the equipment to take the next steps, I needed, at most, MXN$1677. After an exhausting search, I bought everything for only MXN$1226.60, 73% of my budget. I’m posting shorter updates on Twitter, add me.

I made a beautiful, new beverage-can stove! My best ever? Something new: high-temp silicone to seal the seam, so it's pressurized. More efficient. A coin will cover fuel port. Patched my old stove with silicone, too. Silicone cures in 24hrs, then I can (pun not intended!) test it pic.twitter.com/iNQnB98nym

— Hunter Hogan NOW (@HunterHoganNOW) January 13, 2018

Steps to produce psilocybin

  1. Sterilize equipment
  2. Prepare “seed” from fruiting bodies or from spores
  3. Prepare substrate in container
  4. Add the “seed” to the substrate container
  5. Put seeded substrate container into colonizing chamber
  6. When substrate is fully colonized, add sterile water
  7. Put fully colonized substrate container into fruiting chamber
  8. Use spores and fruiting bodies to create new “seed”
  9. Harvest and preserve fruit bodies

Equipment cost and status

Since the first post, I have updated actual costs, researched and revised projected costs, and refined the equipment list. To improve readability, I have broken the items into multiple tables.

Equipment I need

ItemMXN$ImportanceEase to get?Step 123456789Item's options or alternative itemsOther notes
Duct tape$200.00EssentialEasy123456789If I must explain the versatility of duct tape, then you will never understand
Food dehydrator$2,000.00EssentialModerate9This area has high humidity, so air drying is not an option
Fruiting bags, 2$200.00EssentialVery difficult7Real boxesSubstrate container must exchange oxygen without contamination
Fruiting chamber$1,000.00EssentialDifficult7TerrariumDifferent requirements than colonizing chamber; many variables
Fruiting chamber, humidity source$720.00EssentialDifficult7Ultrasonic
Fruiting chamber, hygrometer$500.00EssentialDifficult7Combo thermometer & hygrometerHumidity for fruiting must be ≥95%
Fruiting chamber, temperature control$300.00EssentialVery difficult27Mycelium cell death at 33°C; ideal temperature 20-22°; yield reduced above that
Inoculation loop$60.00HighVery difficult28Flame sterilized paperclip?I feel like this tool is overkill
Inoculation syringe$60.00EssentialDifficult24
Scale$350.00EssentialDifficult2Sensitive to 0.01g
Spore prints, bags$50.00EssentialModerate8
Spore prints, container for making$200.00EssentialModerate8Autoclavable
Storage container (autoclavable)$200.00EssentialDifficult9
Storage, desiccant$200.00EssentialModerate9
Super glue$50.00EssentialEasy123456789
Transportation, projected$200.00EssentialModerate23456789

Some of the equipment is quite expensive, such as the food dehydrator. I want to find alternatives to lower these costs.

Equipment I want

ItemMXN$ImportanceEase to get?Step 123456789Item's options or alternative itemsOther notes
Substrate container, thermometer, 2$400.00UsefulVery difficult57Must autoclave & must read <20°More accurate monitoring
Substrate, organic brown rice flour$0.00UsefulVery difficult3What is the best substrate I can buy in Mexico?
Substrate, rye$0.00UsefulVery difficult3What is the best substrate I can buy in Mexico?

Equipment I have

ItemMXN$ImportanceEase to get?Step 123456789Item's options or alternative itemsOther notes
Agar, potato dextrose$1,370.00EssentialDon't ask2Increases yield, reduces time, easier to detect contamination
Aluminum foil$29.50UsefulEasy2358Real lab equipmentUse in pressure cooker and in making chambers
Cardboard boxes$0.00EssentialEasy24689Real lab equipmentStill air box & other uses
Colonizing chamber$127.00EssentialAnnoying25TerrariumCardboard boxes, pieces of styrofoam ice cooler, plastic wrap, aluminum foil, duct tape
Colonizing chamber, heat source$250.00EssentialModerate5Aquarium heater with thermostat; should help with humidity
Colonizing chamber, humidity source$35.50EssentialModerate5Ultrasonic humidifierTezontle. Aquarium heater in a bucket.
Colonizing chamber, hygrometer$110.00HighDifficult5Humidity for colonization must be ≥95%; analog: unsure of accuracy
Colonizing chamber, thermometer$30.00EssentialEasy25Mycelium cell death at 33°C
Disinfectant spray$51.50HighEasy12456789
Electricity$0.00EssentialAm I homeless?2579
Fruiting chamber, thermometer$33.50EssentialEasy7Mycelium cell death at 33°C; ideal temperature 20-22°; yield reduced above that
Glass jar, 235 ml$13.50EssentialModerate26Autoclavable
Glass jars, 4, ~200ml$0.00UsefulModerate26Autoclavable
Gloves, sterile, 5 pairs, disposable$30.00EssentialEasy2456789Do reusable gloves exist?
Heat mat$380.00UnknownModerateWhat should I do with this? It was the heat source for agar chamber, but no thermostat. Now I've combined agar chamber and colonizing chamber with better heat source.
Lighter$12.50EssentialEasy1236789Real stove
Measuring cup$22.00EssentialEasy236
Measuring spoons$30.00EssentialEasy2
Microporous tape$6.50UsefulEasy123456789Reduce contamination
Petri dishes, glass, 4$152.00EssentialVery difficult2Autoclavable
Plastic wrap$15.50HighEasy24689Real lab equipmentStill air box
Polyfil$25.00EssentialEasy23567Real lab equipmentA poor man's microbe filter
Pressure cooker, 7L$499.50EssentialModerate123679
Pressure cooker, internal stand$17.00UsefulModerate123679Safe & efficient
Silicone sealant, high heat$42.00EssentialVery difficult123689
Spore syringe, 10ml$215.50EssentialVery difficult2
Stove, beverage can$4.00EssentialBad symptoms?1236789Real stoveHeat for pressure cooker
Stove, liquid alcohol fuel$86.00EssentialModerate123689Real stoveMy apartment doesn't have a stove, so I must use my beverage-can camping stove
Stove, solid alcohol$12.00EssentialEasy123689Real stove
Substrate container, 2$354.50EssentialVery difficult3Real bags
Substrate, oats$16.90UnknownEasy3What is the best substrate I can buy in Mexico?
Substrate, rice flour, white$15.40UnknownModerate3What is the best substrate I can buy in Mexico?
Substrate, rye flour$41.00UnknownVery difficult3What is the best substrate I can buy in Mexico?
Substrate, tezontle$89.00EssentialEasy35PerliteHelps increase humidity and protect mycelium from for; not as good as perlite
Substrate, vermiculite$137.50EssentialDifficult3Protect against rot
Substrate, whole grain brown rice$19.80UnknownEasy3What is the best substrate I can buy in Mexico?
Transportation, to date$564.00EssentialModerate123456789
Utility razors$33.50UsefulEasy489Real lab equipmentReduce contamination

Wasted costs

ItemMXN$StatusImportanceEase to get?Step 123456789Item's options or alternative itemsOther notes
Fruiting body samples$250.00SpoiledEssentialFuck you2Time travelSpoiled because I couldn't afford the equipment to use them
Heating element for mugs$21.50HaveUselessMake coffeeWanted to increase heat and humidity, but too crappy to work
Spore printBloodUsedEssentialI almost died2Time travelContaminated by time I used it

Immediate needs

Finding the nutrient substrate was impossible. I bought some alternatives, but I’m still researching how to proceed. I might need to buy some other grain.

I must make at least four new agar plates, so I must have the digital scale. I feel that I should be able to get a better price, though.

I’m using the duct tape and super glue for important things such as the chambers. Therefore, I will buy quality instead of junk.

I’m ambivalent about thermometers in the substrate because I expect the cost to be excessive.

The estimates in the table below are the maximum I would spend.

ItemMXN$StatusImportanceEase to get?Item's options or alternative itemsOther notes
Total$1,200
Duct tape$200NeedEssentialEasyIf I must explain the versatility of duct tape, then you will never understand
Super glue$50NeedEssentialEasy
Transportation, projected$40NeedEssentialModerate
Inoculation loop$60NeedHighVery difficultFlame sterilized paperclip?I feel like this tool is overkill
Scale$350NeedEssentialDifficultSensitive to 0.01g
Substrate, organic brown rice flour$50WantUsefulVery difficultWhat is the best substrate I can buy in Mexico?
Substrate, rye$50WantUsefulVery difficultWhat is the best substrate I can buy in Mexico?
Substrate container, thermometer, 2$400WantUsefulVery difficultMust autoclave & must read <20°More accurate monitoring

Need for cash

MXN$1200 is US$65 according to PayPal. This is my chance to recover. Please support my return to society. PayPal account: [email protected]

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